Medilol

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Extraction and Identification Of Tannins From Cinnamon And Clove

( 2 Votes )

CINNAMON:

BOTANICAL ORIGIN: Cinnamomum loureirii

                     FAMILY: Lauraceae

               PART USED:Dried bark

CLOVE:

BOTANICAL ORIGIN: Eugenia caryophyllus

                     FAMILY: Myrtaceae

               PART USED:Dried flowering bud

THEORY:

Tannin (vegetable tannin, i.e. a type of biomolecule, as opposed to modern synthetic tannin) is an astringent, bitter plant polyphenolic compound that binds to and precipitates proteins and various other organic compounds including amino acids and alkaloids. Tannins have molecular weights ranging from 500 to over 3,000 (Gallic acid esters) and up to 20,000 (proanthocyanidins). Tannins are incompatible with alkalis, gelatin, heavy metals, iron, lime water, metallic salts, strong oxidizing agents and zinc sulfate, since they form complexes and precipitate in aqueous solution.

REQUIREMENTS:

                            Drug extract, Test tubes, Pipette, 5% Ferric chloride solution, Bromine water

PROCEDURE:

Prepared 10% w/v extract by boiling 10g of powdered drug with 100ml of distilled water for 5-10 minutes.Then filtered the extract and carried out the identification tests with the filterate.

USES:

§  Tannins are an important ingredient in the process of tanning leather.

§  Wood adhesive

§  Antiparasitic effects

§  Antibacterial

§  Precipitate proteins

§  Antiviral

 IDENTIFICATION TESTS FOR CLOVE:

Sr

                    EXPERIMENT

          OBSERVATION

          INFERENCE

1.

Took 2ml of extract in a test tube,added 2ml of distilled water and added 1-2 drops of FERRIC CHLORIDE SOLUTION

Bluish black color was appeared

Identification of hydrolysable tannins

2.

Took 2ml of extract in a test tube, added 1-2 drops of  BROMINE WATER.

No change in color

Identification of saturation in hydrolysable tannins

 IDENTIFICATION TESTS FOR CINAMMON:

Sr.

EXPERIMENT

OBSERVATION

INFERENCE

1.

Took 2ml of extract in a test tube, added 2ml of distilled water and added 1-2 drops of FERRIC CHLORIDE SOLUTION

Greenish black color was appeared

Identification of condensed tannins

2.

Took 2ml of extract in a test tube, added 1-2 drops of BROMINE WATER

Dark orange color was appeared

Identification of condensed tannins

Result for clove:

 Clove contains hydrolysable tannins that are pyrogallol tannins.

Result for cinnamon:

Cinnamon contains condensed tannins that are catechol tannins. 

 

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